Layer Origin PureHMO Prebiotic Review (Human Milk Oligosaccharide)

Layer Origin’s PureHMO – Human Milk Oligosaccharide (HMO 2’FL) – is a prebiotic powder supplement that has been clinically studied and has shown to promote healthy gut microflora, improve immune system and support cognitive health.

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You’ve probably heard a lot about probiotics for gut health, but do you know anything about prebiotics? Prebiotics are essentially food for the beneficial bacteria that resides in your gut. Allowing the good bacteria to replace the bad bacteria can help fight against diseases like type 2 diabetes, obesity and a leaky gut. You can also achieve more regular and comfortable bowel movements with the use of prebiotics.

You can source your prebiotics from foods like garlic, bananas, honey and asparagus. There is also something called human milk oligosaccharides that can act as prebiotics for the body. In this article I’m going to explain what these HMO prebiotics are, their benefits and the Layer Origin Pure HMO Prebiotic supplement. I will also answer some of the most frequently asked questions about HMO prebiotics, so let’s get started.

What Are HMO Prebiotics? Human Milk Oligosaccharides Explained

HMO prebiotics, or human milk oligosaccharides, are a type of sugar that is found in human breast milk. These carbohydrates are not easily digestible, so they have the ability to travel to the large intestine and benefit things like your gut microbiome, immune system and brain health. You can only find HMOs in breast milk naturally, but there is also a scientific process that can be used in a lab to create them using enzymes and fermentation. This is beneficial for adults that are looking to reap the benefits of HMO prebiotics as well.

Quick Summary of Layer Origin Pure HMO Prebiotic Review

Product:PureHMO Prebiotic
Brand:Layer Origin
Key Benefits:Could Promote Digestive Health, May Help Support Immune Function, Could Improve Cognitive Function, May Help Promote a Healthy Metabolism
Active Ingredient:Human Milk Oligosaccharide 2’-Fucosyllactose
Where to Buy:Layer Origin Official website
Avg. Customer Rating:4.8/5
GW Rating:4.6/5

Layer Origin PureHMO Prebiotic

Courtney D'Angelo

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Quality of Ingredients Used
Effectiveness of Product
Overall Value


Layer Origin's PureHMO Prebiotic – Human Milk Oligosaccharide (HMO 2'FL) – has shown to significantly promote healthy gut microflora, improve immune system and support cognitive health.


Introducing Pure HMO Prebiotic by Layer Origin

pureHMO prebiotic layer origin

Pure HMO Prebiotic by Layer Origin is a high- super prebiotic is a high-purity human milk oligosaccharide that has gone through the fermentation process. It is designed to provide multiple health benefits such as better digestive health, boosted immune function and optimal brain health. The potent ingredients that are originally found in mother's milk, you actually get more benefits than you would find with traditional oligosaccharides like GOS, FOS, and inulin.

Pure HMO Prebiotic by Layer Origin supports the growth of more than 180 billion CFU probiotics in each serving of product thanks to Bifidobacterium and Akkermansia Muciniphila. This promotes a very healthy gut microbiome that can support your immune function. HMOs tend to be better for your body than prebiotics not sourced from humans. You can use this product to reduce inflammation in the body and fight back against things like pathogens and viruses.

Behind the Brand

Layer Origin was created by two Cornell scientists that were involved with research regarding different methods of making human milk oligosaccharide (HMO) without the use of human milk. During this process, they were intrigued by all of the different health benefits that this unique fiber can provide. This inspired their concept of Layer Origin as a brand that would go on to develop the best line of HMO prebiotic supplements using the most current scientific findings.

Pure HMO Prebiotic and the other supplements sold by Layer Origin are designed to feeds our bodies as well as the bacteria in our gut. This allows for the rebuilding of the bacterial ecosystem within our microbiome regardless of how old we are or what kind of diet we’re following.


  • Supports a healthy metabolism
  • Boosts immune function
  • Creates a more diverse microbiome
  • Supports brain health and cognitive function
  • Created by two Cornell scientists
  • Not actual human milk


  • In some cases, you may experience some degree of abdominal discomfort, bloating or gas as you get used to this type of product

Claimed Benefits

pure HMO benefits

Have I piqued your interest regarding HMO prebiotics, and you would like to learn more about this product from Layer Origin? Here are some of the benefits that the brand claims you can achieve with regular use of their product.

Could Promote Digestive Health

Layer Origin’s Pure HMO Prebiotic has been clinically proven to reduce instances of bloating, gas and constipation. It can relieve stomach pain and discomfort after eating certain trigger foods. It can also help control the symptoms associated with irritable bowel syndrome thanks to a healthier gut ecosystem.

May Help Support Immune Function

Adding Bifidobacteria and Akkermansia to your gut microbiome can create a more diverse environment that will support your immune function. Much of our immune system resides in our gut, so it makes sense to focus on GI health if you’re looking to achieve more protection against things like illness, pathogens and viruses.

Could Improve Cognitive Function

Layer Origin Pure HMO Prebiotic provides nutrients for optimal brain development and maintenance. It can also boost your cognitive functions, so you feel more focused, your memory is better, and you’re energized and alert.

May Help Promote a Healthy Metabolism

When you use a product like Layer Origin Pure HMO Prebiotics, you are also increasing the number of probiotics in the gut. This helps your body better digest your food and boost your metabolism.

Active Ingredient Human Milk Oligosaccharide 2’-Fucosyllactose Analysis

Human Milk Oligosaccharide 2’-Fucosyllactose ingredient

Each serving of Layer Origin Pure HMO Prebiotics contains 1800 milligram of Human Milk Oligosaccharide 2’-Fucosyllactose. This is not sourced from human milk, though it provides all of the same benefits. Human Milk Oligosaccharide 2’-Fucosyllactose is the most abundant oligosaccharides in human milk. The prebiotic effect that comes from supplementation will serve as food for your intestinal bacteria. HMOs have the unique ability to escape the normal digestive process, this allows it to reach the colon. This leads to a more diverse microbiome that will benefit your overall health.

Babies get their HMO prebiotics from their mother’s breast milk. After fat and carbohydrates, these prebiotics are the most prevalent component of breast milk. The purpose of HMOs is to strengthen a baby’s immune system by feeding the beneficial bacteria in the gut and helping the baby develop a healthy microbiome early on. Because of the strong evidence that exists promoting the benefits of HMOs, it makes sense for adults to reap the benefits as well.

Cost and Where to Buy

You can purchase Layer Origin Pure HMO Prebiotics on their website: https://layerorigin.com/. The current cost for 90 capsules is $26.99 for a one-time purchase. You can subscribe to save 15 percent on each order. One serving is three capsules. It’s a relatively affordable product, but you should keep in mind that it may seem like you’re getting a lot of product for your money. However, taking three capsules at once will result in you going through one bottle pretty quickly.

I was also able to find Layer Origin products on Amazon. The Pure HMO supplements can be purchased at similar price points that are featured on the manufacturer’s website. I always like to order right off the brand’s site. This ensures that I’m getting a reputable and authentic product that will provide me with all of the intended benefits. However, feel free to look into other websites and retailers as well if you think that you’re going to get a good deal and you’re confident that you’re getting the real deal.

layer origin pure HMO product image

Frequently Asked Questions on PureHMO

Now it’s time to answer some of the most frequently asked questions regarding the use of Pure HMO as well as HMO prebiotics in general. It’s totally normal to question the formulation of a product before deciding to take the plunge. After all, this is something you’re going to be using on a daily basis and it will be feeding your body.

pureHMO prebiotic powder product

Does This Contain Real Human Milk?

The HMO prebiotics that are included in Layer Origin products do not contain any human milk. They are imitation HMOs that are produced by way of an enzymatic fermentation process. The final product is a powder that is biologically identical to the HMO that is found in human milk. All of the same benefits exist as well.

Can I take PureHMO if I’m Lactose Intolerant, or have a milk allergy?

Research shows that a lot of people that deal with an intolerance to lactose have the ability to tolerate up to 12 grams of lactose in one sitting. This is around one cup of milk. The serving size of lactose inside of Layer Origin Pure HMO is 2.0 grams. This is surely below the limit that you can probably tolerate. However, these trace amounts of lactose will prevent you from being able to consume Pure HMO if you have a severe milk allergy.

Should I take HMO with or without food?

It really doesn’t matter if you take your HMO with or without food. HMOs are not digested by the body the same way that food is. It remains undigested until it reaches the lower intestines. This is where it will be food to the probiotic bacteria in your gut.

Real Layer Origin HMO Prebiotic Reviews and Product Rating

layer origin pure HMO product rating

There are currently 50 reviews listed on the Layer Origin website for their HMO Prebiotic supplement. Eighty-eight percent of the reviews are five out of five-stars. People have found this supplement to be very beneficial in regulating bowel movements, reducing bouts of gas and bloating and eliminating gut sensitivity symptoms. A number of reviews refer to an improvement in energy, sleep quality and brain function.

The only negative reviews that are listed on the Layer Origin website include experiences from people that had a bit of trouble adjusting to the supplement when first starting it. A little bit of bloating or gas is normal as your body adjusts. It’s optimal that you give it enough time to even out before deciding if this supplement is for you or not.

pure HMO layer origin customer reviews

Read more reviews of Pure HMO by Layer Origin here.

The Bottom Line

Gut health is such an important part of your overall health. There are so many different aspects to consider regarding your GI system, so it’s a good idea to assess how you currently feel and think about any health issues or gut issues you’re dealing with.

An HMO prebiotic might be an excellent supplement for you if you’re interested in creating a more diverse microbiome beyond the use of probiotics. If you frequently experience issues like constipation, diarrhea, bloating, gas and a slow digestive process overall, consider using this unique supplement to get back on track.

We know that these HMOs are so important for the development of a baby’s microbiome and immune system. There’s no reason we can’t tap into those benefits as well.

Take a moment to look at Layer Origin’s Pure HMO Prebiotic supplement. It’s a very natural and safe product that does not use any artificial ingredients or fillers. If you’re looking for a place to start, Layer Origin is the brand to consider.

HMO layer origin

Tips for Success from the RD

In addition to taking a supplement that will increase your prebiotic consumption, there are some natural ways that you can achieve this result. Here are my tips for success.

  • There are some very healthy foods that you can work into your diet that have a high prebiotic composition. This includes bananas, asparagus, garlic, artichokes, dandelion greens and onions. See how many you can add into your meals each day.
  • Try to eat as many raw fruits and vegetables as possible. Items that are uncooked tend to have a higher prebiotic composition because this isn’t broken down during any kind of cooking process.
  • Add ingredients such as inulin powder, acacia fiber, chia seeds, flax and pectin into your morning smoothie each day. These are excellent sources of prebiotic fiber that will benefit your overall gut health. Just make sure that you’re choosing one at a time and only starting out with a little bit. Consuming too much of these things all at once can cause some pretty significant bloating and GI upset.
  • Try to work as many different varieties of prebiotics into your diet and routine as possible. We talked a lot about HMO prebiotics in this article, but there are other varieties as well. This includes fructans, galacto-oligosaccharides, starch- and glucose-derived oligosaccharides, pectic oligosaccharide and non-carbohydrate oligosaccharides.
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Courtney D'Angelo, MS, RD

Courtney D'Angelo, MS, RD, earned her masters degree in Nutrition and Foods from the University of Georgia. She's a Registered Dietitian at Morrison Healthcare and has a strong passion in helping people improve their wellness!

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